Refinement methods

- the distinctions between the biological systems and their potential impact on your results - optimised model design for reliable and generalisable results

Clinical Cardiology

- Complications & Unresolved Problems - Possible solutions & difficulties for implementation - Necessity of Research for Novel Therapies

Replacement methods

- existent "organ-on-the-chip" models - induced pluripotent stem cells (iPSc) and organoid systems

Myocardial Infarction

- Ischemia/Reperfusion - Permanent ligature - Cell therapy - Myocardial hydrogels/patches

Systemic interactions

- Heart/Vessels - Kidney - Heart/Vessels - Liver - Systemic disease: obesity, diabetes

Myocardial diseases

- HFpEF models - Aortic banding (TAC) - Diabetes

Atherosclerosis

Models of Atherosclerosis - Wire-injury - Collar Models - Partial Ligature - Stent Implantation

Angiogenesis

- Problems in Clinical Practice that require intervention - Molecular Mechanisms - Clinical Applications of Treatments

Echocardiography (Heart & Vessels)

- Principle of Echocardiography - Echocardiography Applications - Analysis of Echocardiographic Data

Langendorff

- Perfusion and Intraventricular Pressure Measurements - Principles - Technical Execution

Immunohistochemistry

- Principles, Description of Methods, Application in Cardiovascular Diseases Analysis, Troubleshooting - Method of Analysis of Heart and Vessels

Data & Results

- Importance of Interpretation of Data - Presentation of Results - Limitations of Results